Guanidine Hydrochloride Molecular Biology Grade CAS: 50-01-1

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Guanidine Hydrochloride Molecular Biology Grade CAS: 50-01-1

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Product Description

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Overview & Basic Info

Model NO.

C11101/ C11102

CAS Number

50-01-1

EINECS

200-002-3

Grade

Molecular Biology Grade Purity ≥99.5%

Appearance

White Crystal

Content

99.5%

HS Code

2925290090

PH Value (6M)

5.0-5.5

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Product Description

Guanidine hydrochloride is a powerful denaturant and ionizing agent, essential for nucleic acid extraction. It promotes the solubility of hydrophobic molecules and effectively denatures proteins, acting as an inactivator for various enzymes.

In a 6 M solution, it disrupts the ordered secondary structure of proteins by breaking intermolecular non-covalent interactions like hydrogen and hydrophobic bonds. During nucleic acid extraction, it is used in cleaning solutions (> 3M), binding solutions (4-6M) for protein removal, and as a lysate for genomic DNA and total RNA extraction. Notably, it offers low absorbance at A260, A280, and A230, ensuring high-quality nucleic acid products.

CAT NO.	PRODUCT NAME	SIZE	PRICE
C11101	Guanidine hydrochloride	5 KG	$260.00
C11102	Guanidine hydrochloride	25 KG	$999.00

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Technical Specifications

Category	Feature	Specification
Basic Parameters	Formula	CH5N3.HCL
	Molecular Weight	95.53
	Storage	Room temperature, Dry, Dark
	Application	Plasmid extraction, lysis/cleaning solution
Impurity Control	Moisture	≤0.3%
	Ash	≤0.02%
	Iron	≤0.1PPM
	Water Insoluble Matter	≤0.02%
UV Absorption	@ 230 (6M)	≤0.4
	@ 260 (6M)	≤0.04
	@ 280 (6M)	≤0.01
Biological Tests	DNase/RNase (2M)	Not detected
Biological Tests	Extraction Tests	Plasmid & Blood DNA Adopted

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Visual Display

Frequently Asked Questions

What is the primary role of Guanidine Hydrochloride in nucleic acid extraction?

It acts as a powerful denaturant that destroys protein structures and inactivates enzymes, making it ideal for binding solutions and lysates during DNA/RNA extraction.

What is the purity level of this Molecular Biology Grade product?

The product features a high purity level of ≥99.5%, ensuring minimal interference in sensitive laboratory experiments.

How should Guanidine Hydrochloride be stored?

It should be stored at room temperature in a dry, dark place to maintain its stability and prevent hardening over time.

Has this product been tested for nuclease contamination?

Yes, both DNase and RNase tests (at 2M concentration) show that these contaminants are not detected, ensuring safe use for genomic research.

What are the UV absorption specifications for a 6M solution?

The 6M solution has strictly controlled absorbance values: ≤0.4 at 230nm, ≤0.04 at 260nm, and ≤0.01 at 280nm and 320nm.

What applications is this product recommended for?

It is highly recommended for plasmid extraction, blood DNA extraction, protein purification, and creating lysis or cleaning solutions in molecular biology.